Biotechnology

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End date: 2024Has files: Yes

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    To study the genetic diversity and comparative phytochemical analysis in rheum species
    (SRHU, 2024-09-01) Uniyal, Anjali
    Rheum, a valued medicinal herb of Indian Himalayan Region is facing endangerment due to extensive pharmaceutical exploitation, exhibits remarkable antimicrobial, anticancer, antispasmodic, and neuroprotective properties. The present study employs the use of molecular markers, specifically Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeat (ISSR), to assess the genetic diversity of nine Rheum populations across varying altitudes of Uttarakhand region. The Genomic DNA was isolated using a modified CTAB method with few modifications in modified protocol of Doyle and Doyle, DNA was visualized in 0.8% Agarose gel. The isolated DNA was found suitable for the extraction of desired quantity and quality of genomic DNA. The bands showed clear and sharp visible DNA bands DNA quality assessment, using spectrophotometry and gel electrophoresis, precedes genetic diversity analysis. RAPD analysis with 15 primers reveals substantial polymorphism (65.64%) among 142 fragments, emphasizing the plant's genetic diversity. ISSR markers also demonstrate high polymorphism (62.84%) among 189 fragments. The genetic structure, analyzed through Analysis of Molecular Variance (AMOVA), indicates low differentiation among populations. Overall the rresults indicate low population-level genetic diversity (average h = 0.25) but emphasize the species-level diversity. Molecular variance analysis shows minor inter-population differences (5.04% and 2.7%). Dendrogram analysis correlates genetic diversity with altitude, revealing distinct clusters. The maximum Conservation recommendations are proposed based on the obtained genetic data, although marker superiority and reproducibility remain unexplored. The study underscores the suitability of both RAPD and ISSR markers for assessing genetic variability in Rheum species, providing crucial insights for conservation strategies.
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    INVESTIGATION OF POTENTIAL TARGETS AND THEIR INHIBITORS IN MYCOBACTERIUM TUBERCULOSIS
    (SRHU, 2024-09-01) Verma, Ankit
    Mycobacterium tuberculosis (MTB) poses a significant global health threat due to its rapid development of drug resistance, making it difficult to monitor, treat, and prevent tuberculosis (TB) effectively. As a result, scientists have heightened their efforts in exploring novel bioactive substances that possess a unique mechanism of action when combating drug-resistant strains of Mycobacterium tuberculosis. In this study, a high-throughput virtual screening of 30,417 compounds from three independent repositories against putative antitubercular targets, namely MmpL, GlfT2, and MurB, involved in cell-wall biosynthesis was performed. Seventy-five top-ranked inhibitors for further evaluation have been identified.
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    In vitro rapid mass multiplication and molecular validation of Rheum emodi
    (SRHU, 2024-10-01) Upadhyay, Sweta
    India, known for its mega diversity, hosts rich flora with high endemism, particularly in the Northern Himalayas. Among the diverse medicinal plants, Rheum emodi, a highly recognized herb with potent therapeutic properties, faces endangerment due to overexploitation. This study aims to establish a clonal propagation protocol for Rheum emodi using in vitro techniques and assess the genetic fidelity of the regenerated plants through molecular markers.
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    Bioprocess development for the biological removal of iron from subsurface drinking water in Uttarakhand
    (SRHU, 2024-10-01) Rani, Usha
    Uttarakhand is a state in India's northern Himalayas that is blessed with magnificent natural splendour and abundant mountain springs. Even so, during the summer, there is a severe lack of potable water in clusters of hilly villages, there is a severe water shortage, particularly in the summer and in years with less rainfall. In Uttarakhand, the springs serve as the natural source of domestic water. The ionic chemistry of surface and groundwater can vary significantly due to several factors, the most significant of which are urbanization, industrialization, and agricultural development. The strategy for the development and management of water resources as part of the water resources development programme includes an evaluation of the impact that industrialization and urbanization have had on water resources. As a result, in the Haridwar district of Uttarakhand, comprehensive research on the sub-surface and groundwater quality has been carried out to evaluate the water's quality and determine whether or not it is suitable for drinking, residential, or agricultural use.